[The whole genome-wide screening of the longevity-related gene of Uyghur population in Hotan, Xinjiang].

Objective: To discuss the whole genome-wide screening of the longevity-related gene of Uyghur population in Hotan, Xinjiang. Methods: The whole gene scanning using genome-wide association study (GWAS) based on the pooling DNA and the SNP-Map strategy in the longevity group (≥90 years old) and control group (persons who died before 70 years old) was carried out. Then, through detecting varieties of SNP locus, the frequencies of genotype and allele were calculated by sequencing and PCR- restriction fragment length polymorphism (RFLP) method. Results: There was no significant difference of rs6450874, rs6800573 and rs6741735 genotypes and alleles frequency distributions between the two groups, however, there were statistical differences of rs1718307, rs4449651, rs11217987 and rs2907092 genotypes and alleles frequency distributions between the two groups. The TT genotype of rs11217987 (OR=1.462, P=0.034) and the GG genotype of rs2907092 (OR=3.000, P=0.018) were positively correlated to the longevity. And the T allele of rs11217987 (OR=1.382, 95%CI: 1.075-1.776) and the G allele of rs2907092 (OR=1.672, 95%CI: 1.267-2.205) had strong potential function to prolong life. The TT genotype of rs4449651 (OR=6.892, P=0.009) and rs1718307 (OR=4.036, P<0.001) were positively correlated to the longevity. Moreover, the T allele of both SNPs (OR=1.793, 95%CI: 1.298-2.477; OR=1.830, 95%CI: 1.391-2.407) had strong potential function to prolong life. Conclusions: The GWAS test can effectively and extensively analyze human longevity-related genes. And rs1718307, rs4449651, rs11217987 and rs2907092 are related to the longevity of Uyghur population in Hotan, Xinjiang.

[Association of single nucleotide polymorphisms of IL-6 gene with longevity in Uyghurs in Xinjiang].

OBJECTIVE: To investigate whether the variations of genes encoding pro-inflammatory cytokines is associated with longevity in Uyghurs in Xinjiang. METHODS: A total of 240 individuals aged ≥90 years from Xinjiang Uyghur Autonomous Region were enrolled between 1999 and 2012 as the longevity group, 92 unrelated individuals from the same area who died naturally at the age of 59-73 in the same period were selected as the control group. Seven single nucleotide polymorphisms (SNPs) of 4 genes (tumor necrosis factor (TNF)-α(rs1800629), IL-6(rs1800796, rs10499563), C-reactive protein (CRP) (rs2808630, rs1205) and IL-1ß(rs1143623, rs16944)) were genotyped. Logistic regression was applied to assess the association between each individual SNP and longevity in case-control analyses. RESULTS: The distribution of C allele of rs1800796 in the longevity group (0.298) was lower than that in the control group (0.435) with statistical significance (P=0.001). The distributions of the other 6 genetic variations between the longevity group and the control group showed no statistically significant differences (all P>0.05). CT genotype of rs1800796 was positively associated with longevity (Padd=0.002, OR=1.983, 95%CI: 1.296-3.023), while no significant association was noted between other genotypes and longevity. CONCLUSIONS: SNP of rs1800796 of IL-6 gene may be significantly associated with longevity in Uyghurs in Xinjiang. CT genotype of rs1800796 is significantly associated with longevity. C allele of rs1800796 may be a negative factor for longevity.

Interactive association of heat shock protein 70 genes variants with natural longevity in Xinjiang Hetian Uygur ethnicity.

Mounting evidence suggests that all organisms at the cellular level respond to stress by synthesizing heat shock proteins at the expense of other proteins, and the ability of human cells to respond to heat stress decreases with aging. We thus investigate the association of 3 variants (A1267G in HSPA1B, G190C in HSPA1A, and T2437C in HSPA1L) in the heat shock protein 70 (Hsp70) family with natural longevity in a Xinjiang Hetian Uygur population. A case-control study was conducted in 191 healthy individuals greater than 90 years of age, and 53 naturally died persons 65-70 years of age. Promoter activity was evaluated by luciferase reporter assays. The data were analyzed using an EH/EH+ program for haplotype prediction and MDR software for gene-gene interaction. All studied variants satisfied the Hardy-Weinberg equilibrium in each group. In single-locus analysis, no significant differences were found between long-lived people and short-lived people in the genotype/allele distributions of all variants. In contrast, haplotype analysis indicated that haplotypes A-G-C and A-C-T were more prevalent in long-lived people than short-lived people (P=0.026 and 0.017), and the analysis conferred a 3.46- and 4.51-fold increased tendency for longevity, respectively (P=0.025 and 0.016). The haplotype results were strengthened by interaction analysis, which suggests an optimal model in which G190C and T2437C exert an interacting effect on longevity. No functional significance was observed between 190G and 190C alleles in both control and heat-inducible A549 cells (P>0.05). Taken together, our findings suggested that common genetic variants in Hsp70 family might contribute interactively to longevity the Xinjiang Hetian Uygur population.

[Polymorphism of apolipoprotein E gene and natural longevity in the Xinjiang Uighur people: an association study].

OBJECTIVE: To explore the distribution of polymorphisms of apolipoprotein E(apoE) gene and its relation to natural longevity in the Xinjiang Uighur people. METHODS: Using polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP), the authors obtained data from centenarians(n=42), 90-year-old people (n=102), 65-70-year-old people (n=70) and controls(n=53). RESULTS: The frequencies of apoE genotypes epsilon 3/4, epsilon 3/3 and epsilon 2/3 were 2.4%, 69.0% and 23.8% respectively in the centenarian group, and the frequencies of their epsilon 4, epsilon 3 and epsilon 2 alleles were 1.2%, 82.1% and 16.7%. The frequencies of genotype epsilon 3/4 and epsilon 4, epsilon 3 allele in the centenarian group were significantly lower than those in controls(P< 0.01), whereas the frequencies of genotype epsilon 2/3 and epsilon 2 allele in the centenarian group were significantly higher than those in controls(P< 0.01). Longevity was noted to be positively correlated with epsilon 2 and negatively correlated with epsilon 4. CONCLUSION: This relationship reflects a genetic influence on differential survival and may point to pleiotropic age-dependent effects on longevity.